A systematic review and economic evaluation of intraoperative tests [RD-100i one-step nucleic acid amplification (OSNA) system and Metasin test] for detecting sentinel lymph node metastases in breast cancer

Authors

Huxley, N.

Jones-Hughes, T.

Coelho, H.

Snowsill, T.

Cooper, C.

Meng, Y.

Hyde, C.

Mújica-Mota, R.

Published

Jan 2015

Abstract

BACKGROUND: In breast cancer patients, sentinel lymph node biopsy is carried out at the same time as the removal of the primary tumour to postoperatively test with histopathology for regional metastases in the sentinel lymph node. Those patients with positive test results are then operated on 2-4 weeks after primary surgery to remove the lymph nodes from the axilla (axillary lymph node dissection, ALND). New molecular tests RD-100i [one-step nucleic acid amplification (OSNA); based on messenger RNA amplification to identify the cytokeratin-19 (CK19) gene marker] (Sysmex, Norderstedt, Germany) and Metasin (using the CK19 and mammaglobin gene markers) (Cellular Pathology, Princess Alexandra Hospital NHS Trust, Harlow, UK) are intended to provide an intraoperative diagnosis, thereby avoiding the need for postoperative histopathology and, in positive cases, a second operation for ALND. OBJECTIVE: To evaluate the clinical effectiveness and cost-effectiveness of using OSNA and Metasin in the NHS in England for the intraoperative diagnosis of sentinel lymph nodes metastases, compared with postoperative histopathology, the current standard. DATA SOURCES: Electronic databases including MEDLINE, MEDLINE In-Process & Other Non-Indexed Citations, EMBASE, The Cochrane Library and the Health Economic Evaluations Database as well as clinical trial registries, grey literature and conference proceedings were searched up to July 2012. REVIEW METHODS: A systematic review of the evidence was carried out using standard methods. Single-gate studies were used to estimate the accuracy of OSNA with histopathology as the reference standard. The cost-effectiveness analysis adapted an existing simulation model of the long-term costs and health implications of early breast cancer diagnostic outcomes. The model accounted for the costs of an extended first operation with intraoperative testing, the loss of health-related quality of life (disutility) from waiting for postoperative test results, disutility and costs of a second operation, and long-term costs and disutility from lymphoedema related to ALND, adjuvant therapy, locoregional recurrence and metastatic recurrence. RESULTS: A total of 724 references were identified in the searches, of which 17 studies assessing test accuracy were included in the review, 15 on OSNA and two on Metasin. Both Metasin studies were unpublished. OSNA sensitivity of 84.5% [95% confidence interval (CI) 74.7% to 91.0%] and specificity of 91.8% (95% CI 87.8% to 94.6%) for patient nodal status were estimated in a meta-analysis of five studies [unadjusted for tissue allocation bias (TAB)]. At these values and a 20% node-positive rate, OSNA resulted in lifetime discounted cost-savings of £498 and a quality-adjusted life-year (QALY) loss of 0.048 relative to histopathology, that is, £4324 saved per QALY lost. The most favourable plausible scenario for OSNA in terms of the node-positive rate (range 10-40%), diagnostic accuracy values (91.3% sensitivity and 94.2% specificity, from three reports that adjusted for TAB), the costs of histopathology, OSNA and second surgery, and long-term costs and utilities resulted in a maximum saving per QALY lost of £10,500; OSNA sensitivity and specificity would need to be ≥95% for this figure to be ≥£20,000. LIMITATIONS: There is limited evidence on the diagnostic test accuracy of intraoperative tests. The quality of information on costs of resource utilisation during the diagnostic pathway is low and no evidence exists on the disutility of waiting for a second surgery. No comparative studies exist that report clinical outcomes of intraoperative diagnostic tests. These knowledge gaps have more influence on the decision than current uncertainty in the performance of postoperative histopathology in standard practice. CONCLUSIONS: One-step nucleic acid amplification is not cost-effective for the intraoperative diagnosis of sentinel lymph node metastases. OSNA is less accurate than histopathology and the consequent loss of health benefits in this patient group is not compensat d for by health gains elsewhere in the health system that may be obtained with the cost-savings made. The evidence on Metasin is insufficient to evaluate its cost-effectiveness. STUDY REGISTRATION: This study is registered as PROSPERO CRD42012002889. FUNDING: The National Institute for Health Research Health Technology Assessment programme.

Citation

BibTeX citation:
@article{n.2015,
  author = {Huxley, N. and Jones-Hughes, T. and Coelho, H. and Snowsill,
    T. and Cooper, C. and Meng, Y. and Hyde, C. and Mújica-Mota, R.},
  title = {A Systematic Review and Economic Evaluation of Intraoperative
    Tests {{[}RD-100i} One-Step Nucleic Acid Amplification {(OSNA)}
    System and {Metasin} Test{]} for Detecting Sentinel Lymph Node
    Metastases in Breast Cancer},
  journal = {Health Technol Assess},
  volume = {19},
  number = {2},
  date = {2015},
  url = {https://tristansnowsill.co.uk/a-systematic-review-and-economic-evaluation-of-interoperative.html},
  doi = {10.3310/hta19020},
  langid = {en},
  abstract = {BACKGROUND: In breast cancer patients, sentinel lymph node
    biopsy is carried out at the same time as the removal of the primary
    tumour to postoperatively test with histopathology for regional
    metastases in the sentinel lymph node. Those patients with positive
    test results are then operated on 2-4 weeks after primary surgery to
    remove the lymph nodes from the axilla (axillary lymph node
    dissection, ALND). New molecular tests RD-100i {[}one-step nucleic
    acid amplification (OSNA); based on messenger RNA amplification to
    identify the cytokeratin-19 (CK19) gene marker{]} (Sysmex,
    Norderstedt, Germany) and Metasin (using the CK19 and mammaglobin
    gene markers) (Cellular Pathology, Princess Alexandra Hospital NHS
    Trust, Harlow, UK) are intended to provide an intraoperative
    diagnosis, thereby avoiding the need for postoperative
    histopathology and, in positive cases, a second operation for ALND.
    OBJECTIVE: To evaluate the clinical effectiveness and
    cost-effectiveness of using OSNA and Metasin in the NHS in England
    for the intraoperative diagnosis of sentinel lymph nodes metastases,
    compared with postoperative histopathology, the current standard.
    DATA SOURCES: Electronic databases including MEDLINE, MEDLINE
    In-Process \& Other Non-Indexed Citations, EMBASE, The Cochrane
    Library and the Health Economic Evaluations Database as well as
    clinical trial registries, grey literature and conference
    proceedings were searched up to July 2012. REVIEW METHODS: A
    systematic review of the evidence was carried out using standard
    methods. Single-gate studies were used to estimate the accuracy of
    OSNA with histopathology as the reference standard. The
    cost-effectiveness analysis adapted an existing simulation model of
    the long-term costs and health implications of early breast cancer
    diagnostic outcomes. The model accounted for the costs of an
    extended first operation with intraoperative testing, the loss of
    health-related quality of life (disutility) from waiting for
    postoperative test results, disutility and costs of a second
    operation, and long-term costs and disutility from lymphoedema
    related to ALND, adjuvant therapy, locoregional recurrence and
    metastatic recurrence. RESULTS: A total of 724 references were
    identified in the searches, of which 17 studies assessing test
    accuracy were included in the review, 15 on OSNA and two on Metasin.
    Both Metasin studies were unpublished. OSNA sensitivity of 84.5\%
    {[}95\% confidence interval (CI) 74.7\% to 91.0\%{]} and specificity
    of 91.8\% (95\% CI 87.8\% to 94.6\%) for patient nodal status were
    estimated in a meta-analysis of five studies {[}unadjusted for
    tissue allocation bias (TAB){]}. At these values and a 20\%
    node-positive rate, OSNA resulted in lifetime discounted
    cost-savings of £498 and a quality-adjusted life-year (QALY) loss of
    0.048 relative to histopathology, that is, £4324 saved per QALY
    lost. The most favourable plausible scenario for OSNA in terms of
    the node-positive rate (range 10-40\%), diagnostic accuracy values
    (91.3\% sensitivity and 94.2\% specificity, from three reports that
    adjusted for TAB), the costs of histopathology, OSNA and second
    surgery, and long-term costs and utilities resulted in a maximum
    saving per QALY lost of £10,500; OSNA sensitivity and specificity
    would need to be ≥95\% for this figure to be ≥£20,000. LIMITATIONS:
    There is limited evidence on the diagnostic test accuracy of
    intraoperative tests. The quality of information on costs of
    resource utilisation during the diagnostic pathway is low and no
    evidence exists on the disutility of waiting for a second surgery.
    No comparative studies exist that report clinical outcomes of
    intraoperative diagnostic tests. These knowledge gaps have more
    influence on the decision than current uncertainty in the
    performance of postoperative histopathology in standard practice.
    CONCLUSIONS: One-step nucleic acid amplification is not
    cost-effective for the intraoperative diagnosis of sentinel lymph
    node metastases. OSNA is less accurate than histopathology and the
    consequent loss of health benefits in this patient group is not
    compensat d for by health gains elsewhere in the health system that
    may be obtained with the cost-savings made. The evidence on Metasin
    is insufficient to evaluate its cost-effectiveness. STUDY
    REGISTRATION: This study is registered as PROSPERO CRD42012002889.
    FUNDING: The National Institute for Health Research Health
    Technology Assessment programme.}
}
For attribution, please cite this work as:
Huxley, N., Jones-Hughes, T., Coelho, H., Snowsill, T., Cooper, C., Meng, Y., Hyde, C., and Mújica-Mota, R. 2015. “A Systematic Review and Economic Evaluation of Intraoperative Tests [RD-100i One-Step Nucleic Acid Amplification (OSNA) System and Metasin Test] for Detecting Sentinel Lymph Node Metastases in Breast Cancer.” Health Technol Assess 19 (2). https://doi.org/10.3310/hta19020.